Abstract
We report the use of CotC, a major component of the Bacillus subtilis spore coat, as a fusion partner for the expression of two heterologous antigens on the spore coat. Recombinant spores expressing tetanus toxin fragment C (TTFC) of Clostridium tetani or the B subunit of the heat-labile toxin of Escherichia coli (LTB) were used for oral dosing and shown to generate specific systemic and mucosal immune responses in a murine model. This report, expanding the previously described expression of TTFC on the spore surface by fusion to CotB [J Bacteriol 183 (2001) 6294] and its use for oral vaccination [Infect Immun 71 (2003) 2810] shows that different antigens can be successfully presented on the spore coat and supports the use of the spore as an efficient vehicle for mucosal immunisation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Bacterial / analysis
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Antibodies, Bacterial / biosynthesis
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Antigens, Bacterial / chemistry
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Antigens, Bacterial / immunology*
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Bacillus subtilis / chemistry
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Bacillus subtilis / immunology*
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Bacterial Toxins / immunology
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Blotting, Western
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Chromosomes, Bacterial / immunology
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Cyclohexanones / chemistry
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Cyclohexanones / immunology*
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Enterotoxins / immunology
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Enzyme-Linked Immunosorbent Assay
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Escherichia coli Proteins*
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Female
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Genes, Bacterial / genetics
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Immunity, Mucosal / immunology
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Immunization
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Mice
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Mice, Inbred C57BL
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Neutralization Tests
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Peptide Fragments / immunology
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Recombinant Fusion Proteins / immunology
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Spores, Bacterial / chemistry
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Spores, Bacterial / immunology*
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Tetanus / immunology
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Tetanus Toxin / immunology
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Transformation, Bacterial
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Vaccines, Synthetic / chemistry
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Vaccines, Synthetic / immunology
Substances
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Antibodies, Bacterial
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Antigens, Bacterial
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Bacterial Toxins
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Cyclohexanones
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Enterotoxins
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Escherichia coli Proteins
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Peptide Fragments
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Recombinant Fusion Proteins
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Tetanus Toxin
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Vaccines, Synthetic
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tetanus toxin fragment C
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2-crotonyloxymethyl-(4R,5R,6R)-4,5,6-trihydroxycyclohex- 2-enone
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heat-labile enterotoxin, E coli