Increased level of beta-amyloid in the brain of bulbectomized mice

I Yu Aleksandrova; V V Kuvichkin; I A Kashparov; N I Medvinskaya; I V Nesterova; S M Lunin; A N Samokhin; N V Bobkova

doi:10.1023/b:biry.0000018948.04559.ab

Increased level of beta-amyloid in the brain of bulbectomized mice

Biochemistry (Mosc). 2004 Feb;69(2):176-80. doi: 10.1023/b:biry.0000018948.04559.ab.

Authors

I Yu Aleksandrova¹, V V Kuvichkin, I A Kashparov, N I Medvinskaya, I V Nesterova, S M Lunin, A N Samokhin, N V Bobkova

Affiliation

¹ Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia.

PMID: 15000684
DOI: 10.1023/b:biry.0000018948.04559.ab

Abstract

Six weeks after bilateral olfactory bulbectomy, a peptide with molecular weight of 4 kD was revealed in extracts of the neocortex and hippocampus from mice. Using monoclonal antibodies 4G8, this peptide was identified as beta-amyloid. Its level was significantly higher in the bulbectomized animals than in sham-operated mice. The bulbectomized mice displayed sharp impairment in spatial memory when tested in the Morris water maze. The results suggest that bulbectomy initiates in the brain a pathological process similar to human Alzheimer's disease in location, biochemistry, and behavioral manifestations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alzheimer Disease / etiology
Alzheimer Disease / metabolism*
Alzheimer Disease / physiopathology
Amyloid beta-Peptides / analysis
Amyloid beta-Peptides / metabolism*
Animals
Behavior, Animal / physiology
Brain Chemistry*
Disease Models, Animal
Hippocampus / chemistry
Hippocampus / metabolism*
Humans
Male
Maze Learning / physiology
Memory / physiology
Mice
Neocortex / chemistry
Neocortex / metabolism*
Olfactory Pathways / surgery
Peptide Fragments / analysis
Peptide Fragments / metabolism

Substances

Amyloid beta-Peptides
Peptide Fragments