Targeting duplex DNA with DNA-PNA chimeras? Physico-chemical characterization of a triplex DNA-PNA/DNA/DNA

Biopolymers. 2004 Mar;73(4):434-42. doi: 10.1002/bip.10599.

Abstract

Targeting double-stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA-PNA chimeras. A 16-mer 5'-DNA-3'-p-(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and molecular modeling studies. The obtained results showed the formation of a triplex structure having similar, if not slightly higher, stability compared to the same all-DNA complex.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chemical Phenomena
  • Chemistry, Physical
  • Chimera*
  • Circular Dichroism
  • DNA / chemical synthesis
  • DNA / chemistry*
  • DNA / metabolism
  • Hydrogen Bonding
  • Hydrogen-Ion Concentration
  • Models, Molecular
  • Molecular Structure
  • Nucleic Acid Conformation*
  • Oligodeoxyribonucleotides / chemical synthesis
  • Peptide Nucleic Acids / chemical synthesis
  • Peptide Nucleic Acids / chemistry*
  • Peptide Nucleic Acids / metabolism
  • Pyrimidine Nucleotides / chemistry
  • Temperature
  • Thermodynamics

Substances

  • Oligodeoxyribonucleotides
  • Peptide Nucleic Acids
  • Pyrimidine Nucleotides
  • triplex DNA
  • DNA