Abstract
Targeting double-stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA-PNA chimeras. A 16-mer 5'-DNA-3'-p-(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and molecular modeling studies. The obtained results showed the formation of a triplex structure having similar, if not slightly higher, stability compared to the same all-DNA complex.
Copyright 2004 Wiley Periodicals, Inc.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Chemical Phenomena
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Chemistry, Physical
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Chimera*
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Circular Dichroism
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DNA / chemical synthesis
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DNA / chemistry*
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DNA / metabolism
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Hydrogen Bonding
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Hydrogen-Ion Concentration
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Models, Molecular
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Molecular Structure
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Nucleic Acid Conformation*
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Oligodeoxyribonucleotides / chemical synthesis
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Peptide Nucleic Acids / chemical synthesis
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Peptide Nucleic Acids / chemistry*
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Peptide Nucleic Acids / metabolism
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Pyrimidine Nucleotides / chemistry
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Temperature
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Thermodynamics
Substances
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Oligodeoxyribonucleotides
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Peptide Nucleic Acids
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Pyrimidine Nucleotides
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triplex DNA
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DNA