Abstract
Multiprotein bridging factor 1 (MBF1) is known to be a transcriptional co-activator that mediates transcriptional activation by bridging between an activator and a TATA-box binding protein (TBP). We demonstrated that expression of every three MBF1 from Arabidopsis partially rescues the yeast mbf1 mutant phenotype, indicating that all of them function as co-activators for GCN4-dependent transcriptional activation. We also report that each of their subtypes shows distinct tissue-specific expression patterns and responses to phytohormones. These observations suggest that even though they share a similar biochemical function, each MBF1 has distinct roles in various tissues and conditions.
MeSH terms
-
Arabidopsis / genetics*
-
Arabidopsis / metabolism*
-
Arabidopsis Proteins / biosynthesis
-
Arabidopsis Proteins / genetics
-
Arabidopsis Proteins / isolation & purification
-
Basic-Leucine Zipper Transcription Factors
-
DNA, Complementary / analysis
-
DNA, Complementary / genetics
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism
-
Fungal Proteins / genetics
-
Gene Expression Regulation, Plant / genetics
-
Glutathione Transferase / metabolism
-
Macromolecular Substances
-
Molecular Sequence Data
-
Mutation / genetics
-
Nuclear Proteins / genetics
-
Nuclear Proteins / metabolism
-
Open Reading Frames / genetics
-
Plant Growth Regulators / metabolism
-
Plant Growth Regulators / pharmacology
-
Plant Proteins*
-
Protein Binding / genetics
-
Protein Isoforms / biosynthesis
-
Protein Isoforms / genetics
-
Protein Isoforms / isolation & purification
-
Protein Kinases / genetics
-
Protein Kinases / metabolism
-
RNA, Messenger / genetics
-
Saccharomyces cerevisiae Proteins / genetics
-
Saccharomyces cerevisiae Proteins / metabolism
-
Sequence Homology, Amino Acid
-
Sequence Homology, Nucleic Acid
-
TATA-Box Binding Protein / genetics
-
TATA-Box Binding Protein / metabolism
-
Trans-Activators / biosynthesis*
-
Trans-Activators / genetics*
-
Trans-Activators / isolation & purification
-
Transcription Factors / genetics
-
Transcription Factors / metabolism
-
Transcriptional Activation / genetics*
Substances
-
AHBP-1b protein, Arabidopsis
-
Arabidopsis Proteins
-
Basic-Leucine Zipper Transcription Factors
-
DNA, Complementary
-
DNA-Binding Proteins
-
Fungal Proteins
-
MBF1 protein, Arabidopsis
-
MBF1 protein, S cerevisiae
-
Macromolecular Substances
-
Nuclear Proteins
-
Plant Growth Regulators
-
Plant Proteins
-
Protein Isoforms
-
RNA, Messenger
-
Saccharomyces cerevisiae Proteins
-
TATA-Box Binding Protein
-
Trans-Activators
-
Transcription Factors
-
HBP-1b protein, plant
-
Glutathione Transferase
-
Protein Kinases