Abstract
Efficient high-throughput expression of genes in mammalian cells can facilitate large-scale functional genomic studies. Towards this aim, we developed a simple yet powerful method to deliver genes into cells by cationic polymers on the surface of substrates. Transfection can be achieved by directly contacting nucleic acid-cell mixtures with the cationic substrates, e.g. polyethylenimine/collagen-coated wells. This single-step matrix-surface- mediated transfection method, termed 'surfection', can efficiently deliver multiple plasmids into cells and can successfully assay siRNA-mediated gene silencing. This technology represents the easiest method to transfer combinations of genes in large-scale arrays, and is a versatile tool for live-cell imaging and cell-based drug screening.
MeSH terms
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Animals
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Carrier Proteins / genetics
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Cell Line
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Cell Line, Tumor
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Collagen / chemistry
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DNA / chemistry
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DNA / genetics
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Green Fluorescent Proteins
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Humans
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Luciferases / genetics
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Luciferases / metabolism
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Nerve Tissue Proteins / genetics
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Phosphoproteins / genetics
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Plasmids / chemistry
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Plasmids / genetics
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Polyethyleneimine / chemistry
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RNA / chemistry
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RNA / genetics
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RNA Interference
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RNA-Binding Proteins
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Reproducibility of Results
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Sensitivity and Specificity
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Transfection / instrumentation
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Transfection / methods*
Substances
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Carrier Proteins
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EBNA1BP2 protein, human
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Luminescent Proteins
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Nerve Tissue Proteins
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Phosphoproteins
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RNA-Binding Proteins
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Recombinant Fusion Proteins
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collapsin response mediator protein-1
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Green Fluorescent Proteins
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RNA
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Polyethyleneimine
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Collagen
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DNA
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Luciferases