The time course of the glucose isomerization was investigated. At equilibrium the fructose content in the reaction mixture was 52% of the initial glucose concentration. The equilibrium constants K1 and K-1 for the isomerization reaction were determined at 60 degrees C. Two graphical plots were developed to reduce the problems of determining the individual constant. These plot methods for chemical reaction kinetics were applied with equal validity to the kinetics of glucose isomerization process.