Direct sequencing and bidirectional allele specific polymerase chain reaction of the bovine beta-casein B variant

Anim Genet. 1992;23(6):561-5. doi: 10.1111/j.1365-2052.1992.tb00180.x.

Abstract

We have used the polymerase chain reaction (PCR) to amplify exon VII of the bovine beta-casein gene. The mutations responsible for the B variant were identified by direct sequencing of the amplification products. A bidirectional allele-specific PCR method (BAS-PCR) has been developed using oligonucleotides overlapping the mutation site at their 3' ends. This new procedure allows a rapid and reliable discrimination between the B and non-B alleles of beta-casein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Base Sequence
  • Caseins / genetics*
  • Cattle / genetics*
  • DNA / genetics
  • DNA Mutational Analysis
  • Evaluation Studies as Topic
  • Exons
  • Female
  • Genetic Variation
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*

Substances

  • Caseins
  • DNA