Effect of overexpression of Actinobacillus succinogenes phosphoenolpyruvate carboxykinase on succinate production in Escherichia coli

Appl Environ Microbiol. 2004 Feb;70(2):1238-41. doi: 10.1128/AEM.70.2.1238-1241.2004.

Abstract

Succinate fermentation was investigated in Escherichia coli strains overexpressing Actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). In E. coli K-12, PEPCK overexpression had no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant E. coli strain K-12 ppc::kan, PEPCK overexpression increased succinate production 6.5-fold.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Actinobacillus / enzymology*
  • Actinobacillus / genetics*
  • Culture Media
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Escherichia coli / growth & development
  • Fermentation
  • Genetic Engineering / methods
  • Mutation
  • Phosphoenolpyruvate Carboxykinase (ATP) / genetics
  • Phosphoenolpyruvate Carboxykinase (ATP) / metabolism*
  • Phosphoenolpyruvate Carboxylase / genetics
  • Phosphoenolpyruvate Carboxylase / metabolism
  • Succinic Acid / metabolism*

Substances

  • Culture Media
  • Succinic Acid
  • Phosphoenolpyruvate Carboxylase
  • Phosphoenolpyruvate Carboxykinase (ATP)