Objective: To elucidate the histocompatibility of tissue engineered rat skin through studying the effect of TNF-alpha and IL-2 in immunological rejection after rat skin allograft.
Methods: Tissue engineered skin that the basic materials were taken from neonatal SD rats was cultured in lab, grafted to adult Wistar rats. The expressions of TNF-alpha and IL-2 in grafted tissue were detected with immunohistochemical staining and Western blot.
Results: The expressions of TNF-alpha and IL-2 were remarkable in skin allograft group, but low in tissue engineered skin group.
Conclusion: The expressions of TNF-alpha and IL-2 and the immunological rejection were closely related after skin allograft, but the tissue engineered skin has favorable histocompatibility and doesn't arose obvious immunological rejections.