Evaluation of DNA damage in Dicentrarchus labrax sperm following cryopreservation

L Zilli; R Schiavone; V Zonno; C Storelli; S Vilella

doi:10.1016/j.cryobiol.2003.10.002

Evaluation of DNA damage in Dicentrarchus labrax sperm following cryopreservation

Cryobiology. 2003 Dec;47(3):227-35. doi: 10.1016/j.cryobiol.2003.10.002.

Authors

L Zilli¹, R Schiavone, V Zonno, C Storelli, S Vilella

Affiliation

¹ Laboratory of General and Comparative Physiology, Department of Biological and Environmental Sciences and Technologies, University of Lecce, Via Provinciale Lecce-Monteroni, Lecce 73100, Italy. loredana.zilli@unile.it

PMID: 14697734
DOI: 10.1016/j.cryobiol.2003.10.002

Abstract

In this paper, DNA laddering analysis and single-cell gel electrophoresis (SCGE) or Comet assay, were used to detect DNA damage in response to a cryopreservation process in sea bass spermatozoa. The results obtained demonstrate that the cryopreservation protocol used to cryopreserve the sea bass sperm cause significantly damage at DNA level. In fact, the degree of DNA damage in frozen-thawed sperm (%DNAT=38.2+/-11.2, MT=498.9+/-166.4, n=3) was different (P<0.01) from that measured in fresh sperm (%DNAT=32.7+/-11.1, MT=375.2+/-190.7, n=3). Data here reported also demonstrated the fundamental role played by cryoprotectants (BSA and Me2SO) in reducing fish sperm DNA fragmentation. Finally, from our results, the ability of SCGE to reveal DNA fragmentation in fish sperm is also confirmed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aquaculture
Bass*
Comet Assay
Cryopreservation*
DNA Damage*
DNA Fragmentation
Male
Semen Preservation / methods*
Spermatozoa / pathology*