A novel chemiluminescent method for determination of phloroglucinol

Luminescence. 2003 Nov-Dec;18(6):318-23. doi: 10.1002/bio.740.

Abstract

It was found that the inhibition and enhancement by phloroglucinol of the chemiluminescence from the luminol-K3Fe(CN)6 system were dependent on the pH of luminol solution and the concentration of phloroglucinol. In Na2CO(3)-NaHCO3 buffer, phloroglucinol exhibited strong chemiluminescent enhancement at pH 9.4. On this basis, a flow injection method was developed for the determination of phloroglucinol. The method was simple, rapid, convenient and sensitive, with a detection limit of 2.0 x 10(-9) mol/L. It is effective for determining phloroglucinol in the range of 1.0 x 10(-5)-5.0 x 10(-9) mol/L. The relative standard deviation is 1.3% within one day and 3.2% between days for the determination of 5.0 x 10(-7) mol/L phloroglucinol. The method has been successfully used to determine phloroglucinol in environmental water, with satisfactory results.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Flow Injection Analysis / methods*
  • Hydrogen-Ion Concentration
  • Luminescent Measurements*
  • Luminol / chemistry
  • Phloroglucinol / analysis*
  • Water Pollutants / analysis

Substances

  • Water Pollutants
  • Luminol
  • Phloroglucinol