Decolorization of azo dyes by Rhodobacter sphaeroides

Biotechnol Lett. 2003 Nov;25(21):1815-8. doi: 10.1023/a:1026244909758.

Abstract

Rhodobacter sphaeroides AS1.1737 decolorized more than 90% of several azo dyes (200 mg dyes l(-1)) in 24 h. The optimal culture conditions were: anaerobic illumination (1990 1x), peptone as carbon source, temperature 35-40 degrees C and pH 7-8. Intracellular crude enzyme from this strain had azoreductase activity, optimized temperature as 45-50 degrees C, and decolorization kinetics which were consistent with a ping-pong mechanism.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azo Compounds / pharmacokinetics*
  • Biodegradation, Environmental
  • Color*
  • Coloring Agents / pharmacokinetics*
  • Hydrogen-Ion Concentration
  • Industrial Waste / prevention & control
  • NADH, NADPH Oxidoreductases / chemistry
  • NADH, NADPH Oxidoreductases / metabolism*
  • Nitroreductases
  • Rhodobacter sphaeroides / enzymology
  • Rhodobacter sphaeroides / growth & development*
  • Rhodobacter sphaeroides / metabolism*
  • Temperature
  • Water Pollutants, Chemical / pharmacokinetics*
  • Water Purification / methods*

Substances

  • Azo Compounds
  • Coloring Agents
  • Industrial Waste
  • Water Pollutants, Chemical
  • NADH, NADPH Oxidoreductases
  • Nitroreductases
  • azoreductase