Abstract
Rhodobacter sphaeroides AS1.1737 decolorized more than 90% of several azo dyes (200 mg dyes l(-1)) in 24 h. The optimal culture conditions were: anaerobic illumination (1990 1x), peptone as carbon source, temperature 35-40 degrees C and pH 7-8. Intracellular crude enzyme from this strain had azoreductase activity, optimized temperature as 45-50 degrees C, and decolorization kinetics which were consistent with a ping-pong mechanism.
Publication types
-
Comparative Study
-
Evaluation Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Azo Compounds / pharmacokinetics*
-
Biodegradation, Environmental
-
Color*
-
Coloring Agents / pharmacokinetics*
-
Hydrogen-Ion Concentration
-
Industrial Waste / prevention & control
-
NADH, NADPH Oxidoreductases / chemistry
-
NADH, NADPH Oxidoreductases / metabolism*
-
Nitroreductases
-
Rhodobacter sphaeroides / enzymology
-
Rhodobacter sphaeroides / growth & development*
-
Rhodobacter sphaeroides / metabolism*
-
Temperature
-
Water Pollutants, Chemical / pharmacokinetics*
-
Water Purification / methods*
Substances
-
Azo Compounds
-
Coloring Agents
-
Industrial Waste
-
Water Pollutants, Chemical
-
NADH, NADPH Oxidoreductases
-
Nitroreductases
-
azoreductase