Population data concerning the D1S80 system based on the analysis of 775 persons from Central Poland region was presented. Accordance with the Hardy and Weinberg equilibrium (HWE) was proved using two different statistical approaches. The parameters for the appropriateness of this marker for investigations in the analysed population was also assessed: heterozygosity--0.79, PIC--0.77, PD--0.93, PE--0.62, PI--12.8 and frequency of mutation events--0.27%.