Construction of a genetically modified wine yeast strain expressing the Aspergillus aculeatus rhaA gene, encoding an alpha-L-rhamnosidase of enological interest

Appl Environ Microbiol. 2003 Dec;69(12):7558-62. doi: 10.1128/AEM.69.12.7558-7562.2003.

Abstract

The Aspergillus aculeatus rhaA gene encoding an alpha-L-rhamnosidase has been expressed in both laboratory and industrial wine yeast strains. Wines produced in microvinifications, conducted using a combination of the genetically modified industrial strain expressing rhaA and another strain expressing a beta-glucosidase, show increased content mainly of the aromatic compound linalool.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyclic Monoterpenes
  • Aspergillus / enzymology
  • Aspergillus / genetics*
  • Biotechnology / methods
  • Fermentation
  • Genetic Engineering / methods
  • Glycoside Hydrolases / genetics*
  • Glycoside Hydrolases / metabolism*
  • Monoterpenes / metabolism
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Vitis / metabolism
  • Vitis / microbiology
  • Wine / microbiology*
  • beta-Glucosidase / genetics
  • beta-Glucosidase / metabolism

Substances

  • Acyclic Monoterpenes
  • Monoterpenes
  • linalool
  • Glycoside Hydrolases
  • beta-Glucosidase
  • alpha-L-rhamnosidase