Objective: To clarify the nature of human hepatocyte DNA synthetic stimulated factor (HDSSF) and to lay a foundation for its gene cloning and expression.
Methods: We amplified the target gene fragment by degenerating PCR, labelled the fragment as a probe to screen human fetal liver cDNA library, and then analyzed the HDSSF gene sequence.
Results: A 600 base pair fragment was obtained and labelled successfully with DIG. Human HDSSF sequence was determined by screening the library and gene sequencing. Its cDNA chain was composed of 748 base pairs including 594 base pair open read-code frame and 5' and 3' terminal noncode district sequence.
Conclusion: By screening cDNA library, the HDSSF gene cloning has been gained successfully. HDSSF should be a new hepatocyte growth substance which consists of 198 amino acid residues.