Abstract
Recombinant Pyrococcus furiosus ferredoxin with a [Fe3S4]-cluster was crystallized through steps of optimization and X-ray diffraction data were collected from several crystal forms. Flat plate-like crystals were grown by hanging-drop vapour diffusion. The precipitant used was 30% PEG 400; the pH was varied between 7.0 and 8.0 and hexaamminecobalt(III) chloride was essential for crystal formation. The best crystals belong to space group P2(1), with unit-cell parameters a = 31.23, b = 47.51, c = 52.03 A, beta = 103.86 degrees, and diffract to 1.5 A resolution.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Archaeal Proteins / chemistry*
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Archaeal Proteins / genetics
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Archaeal Proteins / metabolism
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Crystallization / methods
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Crystallography, X-Ray / statistics & numerical data
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Escherichia coli / metabolism
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Ferredoxins / chemistry*
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Ferredoxins / genetics
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Ferredoxins / metabolism
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Iron-Sulfur Proteins / chemistry*
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Iron-Sulfur Proteins / genetics
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Iron-Sulfur Proteins / metabolism
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Neomycin / metabolism
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Pyrococcus furiosus / chemistry*
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
Substances
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Archaeal Proteins
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Ferredoxins
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Iron-Sulfur Proteins
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Recombinant Proteins
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Neomycin