Crystallization of [Fe3S4]-ferredoxin from the hyperthermophile archaeon Pyrococcus furiosus

Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2325-7. doi: 10.1107/s0907444903021267. Epub 2003 Nov 27.

Abstract

Recombinant Pyrococcus furiosus ferredoxin with a [Fe3S4]-cluster was crystallized through steps of optimization and X-ray diffraction data were collected from several crystal forms. Flat plate-like crystals were grown by hanging-drop vapour diffusion. The precipitant used was 30% PEG 400; the pH was varied between 7.0 and 8.0 and hexaamminecobalt(III) chloride was essential for crystal formation. The best crystals belong to space group P2(1), with unit-cell parameters a = 31.23, b = 47.51, c = 52.03 A, beta = 103.86 degrees, and diffract to 1.5 A resolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Archaeal Proteins / chemistry*
  • Archaeal Proteins / genetics
  • Archaeal Proteins / metabolism
  • Crystallization / methods
  • Crystallography, X-Ray / statistics & numerical data
  • Escherichia coli / metabolism
  • Ferredoxins / chemistry*
  • Ferredoxins / genetics
  • Ferredoxins / metabolism
  • Iron-Sulfur Proteins / chemistry*
  • Iron-Sulfur Proteins / genetics
  • Iron-Sulfur Proteins / metabolism
  • Neomycin / metabolism
  • Pyrococcus furiosus / chemistry*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism

Substances

  • Archaeal Proteins
  • Ferredoxins
  • Iron-Sulfur Proteins
  • Recombinant Proteins
  • Neomycin