Molecular cloning of an epidermal growth factor-like toxin and two sodium channel toxins from the sea anemone Stichodactyla gigantea

Tomohiro Honma; Hiroshi Nagai; Yuji Nagashima; Kazuo Shiomi

doi:10.1016/j.bbapap.2003.08.007

Molecular cloning of an epidermal growth factor-like toxin and two sodium channel toxins from the sea anemone Stichodactyla gigantea

Biochim Biophys Acta. 2003 Dec 1;1652(2):103-6. doi: 10.1016/j.bbapap.2003.08.007.

Authors

Tomohiro Honma¹, Hiroshi Nagai, Yuji Nagashima, Kazuo Shiomi

Affiliation

¹ Department of Food Science and Technology, Tokyo University of Fisheries, Konan-4, Minato, Tokyo 108-8477, Japan.

PMID: 14644045
DOI: 10.1016/j.bbapap.2003.08.007

Abstract

An epidermal growth factor (EGF)-like toxin (gigantoxin I) and two sodium channel toxins (gigantoxins II and III), previously isolated from the sea anemone Stichodactyla gigantea, were cloned for their cDNAs. The precursor protein of gigantoxin I is composed of a signal peptide, propart and mature peptide, similar to those of gigantoxins II and III, and is much simpler in structure than those of mammalian EGFs. In addition, gigantoxin I as well as gigantoxins II and III was demonstrated to be contained in nematocysts, suggesting that gigantoxin I functions as a toxin in S. gigantea.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Cnidarian Venoms / chemistry
Cnidarian Venoms / genetics*
DNA, Complementary / analysis
Epidermal Growth Factor / chemistry
Epidermal Growth Factor / genetics*
Molecular Sequence Data
Peptide Fragments / chemistry
Peptide Fragments / genetics*
Sea Anemones / physiology*
Sequence Alignment
Sodium Channels / chemistry
Sodium Channels / genetics*
Toxins, Biological / chemistry
Tumor Cells, Cultured

Substances

Cnidarian Venoms
DNA, Complementary
Peptide Fragments
Sodium Channels
Toxins, Biological
Epidermal Growth Factor

Associated data

GENBANK/AB110012
GENBANK/AB110014
GENBANK/AB110015