A new method of establishing adult rat model of skin necrosis using L-arginine (L-ARG) is described. The adult rats were subjected to 5 to 7-day treatment with L-ARG at the dose of 500 mg/kg.b.w.d that was dissolved in the drinking water, and skin necrosis was subsequently observed in approximately 50% of the rats. Using L-ARG is simple and efficient to establish animal models of skin necrosis, yielding a high success rate within a relatively shorter period of time.