Abstract
13C-Labeled and unlabeled iodoacetanilides have been synthesized for covalent modification of the sulfhydryl groups of cysteine residues in proteins or peptides. A combination of these reagents, coupled with mass spectrometry, is a powerful tool for quantitative analysis of peptides and hence proteins.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetanilides / chemical synthesis*
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Acetanilides / chemistry
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Amino Acid Sequence
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Carbon Isotopes
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Cysteine / chemistry
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Hydrocarbons, Iodinated / chemical synthesis*
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Hydrocarbons, Iodinated / chemistry
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Isotope Labeling / methods
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Peptides / analysis*
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Peptides / chemistry
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
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Sulfhydryl Compounds / chemistry
Substances
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Acetanilides
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Carbon Isotopes
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Hydrocarbons, Iodinated
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Peptides
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Sulfhydryl Compounds
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Cysteine