Synthesis of 13C-labeled iodoacetanilide and application to quantitative peptide analysis by isotope differential mass spectrometry

Bioorg Med Chem Lett. 2003 Sep 1;13(17):2913-6. doi: 10.1016/s0960-894x(03)00503-1.

Abstract

13C-Labeled and unlabeled iodoacetanilides have been synthesized for covalent modification of the sulfhydryl groups of cysteine residues in proteins or peptides. A combination of these reagents, coupled with mass spectrometry, is a powerful tool for quantitative analysis of peptides and hence proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetanilides / chemical synthesis*
  • Acetanilides / chemistry
  • Amino Acid Sequence
  • Carbon Isotopes
  • Cysteine / chemistry
  • Hydrocarbons, Iodinated / chemical synthesis*
  • Hydrocarbons, Iodinated / chemistry
  • Isotope Labeling / methods
  • Peptides / analysis*
  • Peptides / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Sulfhydryl Compounds / chemistry

Substances

  • Acetanilides
  • Carbon Isotopes
  • Hydrocarbons, Iodinated
  • Peptides
  • Sulfhydryl Compounds
  • Cysteine