Stabilizing effect of artificial stabilizers for binding activity of QCM immunosensors

IEEE Trans Ultrason Ferroelectr Freq Control. 2003 Oct;50(10):1234-5. doi: 10.1109/tuffc.2003.1244737.

Abstract

We compare stabilizing effects of artificial and natural stabilizers for maintenance of binding activity of immobilized antibody on the gold surface of quartz crystal microbalance (QCM). Binding activity between immobilizing antibody and specific antigen was measured and considered as a calculated frequency shift with QCM-immunosensor after temperature increasing and long-term storage. According to the experimental results, one stabilizer among eight samples shows the best stabilizing effect for QCM-immunosensor.

Publication types

  • Comparative Study
  • Evaluation Study
  • Letter
  • Validation Study

MeSH terms

  • Adsorption
  • Antigen-Antibody Complex / analysis*
  • Antigen-Antibody Complex / chemistry*
  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • Coated Materials, Biocompatible / chemistry*
  • Drug Stability
  • Electrochemistry / instrumentation*
  • Electrochemistry / methods
  • Equipment Failure Analysis
  • Immunoassay / instrumentation*
  • Immunoassay / methods
  • Microchemistry / instrumentation*
  • Microchemistry / methods
  • Protein Binding
  • Quartz
  • Temperature

Substances

  • Antigen-Antibody Complex
  • Coated Materials, Biocompatible
  • Quartz