Abstract
3-hydroxyphenylacetylene (3-HPA) served as a novel, activity-dependent, fluorogenic and chromogenic probe for bacterial enzymes known to degrade toluene via meta ring fission of the intermediate, 3-methylcatechol. By this direct physiological analysis, cells grown with an aromatic substrate to induce the synthesis of toluene-degrading enzymes were fluorescently labeled.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Acetylene / analogs & derivatives*
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Acetylene / metabolism
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Burkholderia cepacia / enzymology
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Burkholderia cepacia / metabolism*
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Catechols / metabolism*
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Fluorescent Dyes / metabolism*
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Microscopy, Fluorescence
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Pseudomonas / enzymology
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Pseudomonas / metabolism*
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Ralstonia / enzymology
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Ralstonia / metabolism*
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Toluene / metabolism*
Substances
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Catechols
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Fluorescent Dyes
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3-methylcatechol
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Toluene
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Acetylene