Comparison of PCR-enzyme-linked immunosorbent assay and real-time PCR assay for diagnosis of an unusual case of cerebral toxoplasmosis in a stem cell transplant recipient

J Clin Microbiol. 2003 Nov;41(11):5313-6. doi: 10.1128/JCM.41.11.5313-5316.2003.

Abstract

A PCR-enzyme-linked immunosorbent assay and a real-time PCR assay were compared for diagnosis and follow-up of cerebral toxoplasmosis in a stem cell transplant recipient. The sensitivity of detection was similar for both assays but was higher when the assays were performed on buffy coat rather than on whole blood or serum.

Publication types

  • Case Reports
  • Comparative Study

MeSH terms

  • Adult
  • Animals
  • Antibodies, Protozoan / blood
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Immunoglobulin G / blood
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / therapy
  • Male
  • Polymerase Chain Reaction / methods*
  • Stem Cell Transplantation / adverse effects*
  • Toxoplasma / classification
  • Toxoplasma / isolation & purification*
  • Toxoplasmosis, Cerebral / blood
  • Toxoplasmosis, Cerebral / diagnosis*

Substances

  • Antibodies, Protozoan
  • Immunoglobulin G