The advanced glycation end-products (AGEs) are the compounds formed and accumulated in the organism. There is heterogenous group of the glycation products, which have great variety of structural and functional properties. The glycation end-products can be used as markers of the glycation process. Because of the great heterogeneity of the AGEs there is no specific test for their adequate measurement. In this paper there are presented the chromatographic, colorimetric, spectroscopic, mass-spectrometric and serological methods, which are used for determination of AGEs in biological samples. New procedures for the preparation of the model AGEs have been also described. Special attention has been paid to the immunochemical tests. For further assays the model AGE antigens have to be prepared.