Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)

Genet Mekuria; Sunita A Ramesh; Evita Alberts; Terry Bertozzi; Michelle Wirthensohn; Graham Collins; Margaret Sedgley

doi:10.1016/j.jviromet.2003.08.014

Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis)

J Virol Methods. 2003 Dec;114(1):65-9. doi: 10.1016/j.jviromet.2003.08.014.

Authors

Genet Mekuria¹, Sunita A Ramesh, Evita Alberts, Terry Bertozzi, Michelle Wirthensohn, Graham Collins, Margaret Sedgley

Affiliation

¹ School of Agriculture and Wine, The University of Adelaide, Waite Campus, PMB #1, Glen Osmond, SA 5064, Australia.

PMID: 14599680
DOI: 10.1016/j.jviromet.2003.08.014

Abstract

A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Enzyme-Linked Immunosorbent Assay / methods*
Ilarvirus / isolation & purification*
Plant Diseases / virology*
Plant Leaves / virology
Prunus / virology*
RNA, Viral / isolation & purification
Reverse Transcriptase Polymerase Chain Reaction*
Sensitivity and Specificity

Substances

RNA, Viral