[Rac subfamily expression and activity in gastrointestinal cancer cell lines]

Zhonghua Zhong Liu Za Zhi. 2003 Sep;25(5):441-4.
[Article in Chinese]

Abstract

Objective: To investigate the significance of Rac subfamily members in the gastrointestinal carcinogenesis and progression.

Methods: The mRNA expression of Rac1, Rac2 and Rac3 in 12 kinds of gastrointestinal cancer cell lines was examined by semi-quantitative RT-PCR. The activities of Rac1 protein in 5 kinds of gastric cancer cell lines were tested by pull-down assay.

Results: Compared with the normal gastric mucosa and intestinal epithelial cell line, the mRNA expression of Rac1 and Rac3 was up-regulated in most of gastrointestinal cancer cell lines. The activities of Rac1 protein increased markedly in gastric cancer cell lines.

Conclusion: The increased mRNA expression of Rac1 and Rac3 in gastrointestinal cancer cell lines and the abnormal activation of Rac1 protein in gastric cancer cell lines might be correlated with the carcinogenesis of gastrointestinal cancer.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Gastrointestinal Neoplasms / metabolism*
  • Humans
  • RAC2 GTP-Binding Protein
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • rac GTP-Binding Proteins / genetics*
  • rac1 GTP-Binding Protein / analysis
  • rac1 GTP-Binding Protein / genetics*

Substances

  • RAC3 protein, human
  • RNA, Messenger
  • rac GTP-Binding Proteins
  • rac1 GTP-Binding Protein