Preparation of 6-thioguanosine phosphoramidite for oligoribonucleotide synthesis

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1247-9. doi: 10.1081/NCN-120022847.

Abstract

6-Thioguanosine phosphoramidite was prepared, using 2,4-dinitrophenyl as protection group for thio-function, and its stability towards conditions of RNA synthesis was investigated. The results show that the monomer was stable under the conditions of RNA synthesis and suitable for incorporation of thioguanine into oligoribonucleotides.

MeSH terms

  • Amides / chemistry
  • Drug Stability
  • Guanosine / analogs & derivatives*
  • Guanosine / chemical synthesis*
  • Indicators and Reagents
  • Molecular Structure
  • Oligoribonucleotides / chemical synthesis*
  • Phosphoric Acids / chemistry
  • Thionucleosides / chemical synthesis*

Substances

  • Amides
  • Indicators and Reagents
  • Oligoribonucleotides
  • Phosphoric Acids
  • Thionucleosides
  • Guanosine
  • phosphoramidic acid
  • 6-thioguanosine