Activating transcription factor 3 mRNA is upregulated in primary cultures of trigeminal ganglion neurons

Brain Res Mol Brain Res. 2003 Oct 21;118(1-2):156-9. doi: 10.1016/s0169-328x(03)00335-8.

Abstract

Activating transcription factor 3 (ATF3) has been used in as a marker of nerve injury in primary sensory neurons. The purpose of the present studies was to determine whether primary sensory ganglia in culture express ATF3 and, thus, an injured phenotype. At all time points post-plating (1 h-14 days), neurons in culture expressed ATF3 compared to undetectable expression in native ganglia. In addition, NGF was unable to rescue this injured phenotype. Thus, sensory neurons in culture represent a potential model of injured neurons.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Activating Transcription Factor 3
  • Animals
  • Cells, Cultured
  • Male
  • Models, Biological
  • Nerve Growth Factor / pharmacology
  • Neurons, Afferent / cytology
  • Neurons, Afferent / drug effects
  • Neurons, Afferent / metabolism*
  • Peripheral Nerve Injuries*
  • Peripheral Nerves / cytology
  • Peripheral Nerves / metabolism
  • Phenotype
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Reaction Time / drug effects
  • Reaction Time / physiology
  • Time Factors
  • Transcription Factors / genetics*
  • Trigeminal Ganglion / cytology
  • Trigeminal Ganglion / drug effects
  • Trigeminal Ganglion / metabolism*
  • Up-Regulation / drug effects
  • Up-Regulation / genetics

Substances

  • Activating Transcription Factor 3
  • Atf3 protein, rat
  • RNA, Messenger
  • Transcription Factors
  • Nerve Growth Factor