Consecutive analysis of bacterial PCR samples on a single electronic microarray

J Microbiol Methods. 2003 Nov;55(2):471-4. doi: 10.1016/s0167-7012(03)00168-4.

Abstract

For routine mass screening, the use of microarrays is hampered because one chip can only analyze one sample. 16S rRNA gene PCR products of several bacterial strains or mixtures thereof were consecutively loaded on a single electronic microarray and successfully analyzed using probes specific for the bacterial strains.

MeSH terms

  • DNA, Bacterial / analysis*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics
  • Nanotechnology / methods*
  • Oligonucleotide Array Sequence Analysis / instrumentation
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymerase Chain Reaction
  • Pseudomonas aeruginosa / genetics
  • RNA, Ribosomal, 16S / analysis*
  • RNA, Ribosomal, 16S / genetics
  • Staphylococcus aureus / genetics

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 16S