Evaluation of the ability of primary selective enrichment to resuscitate heat-injured and freeze-injured Listeria monocytogenes cells

Appl Environ Microbiol. 1992 Sep;58(9):3177-9. doi: 10.1128/aem.58.9.3177-3179.1992.

Abstract

Resuscitation rates of injured Listeria monocytogenes on conventional selective Listeria enrichment broth and nonselective Trypticase soy broth containing 0.6% yeast extract were compared. Cells were heated to 60 degrees C for 5 min or frozen at -20 degrees C for 7 days. Inoculation of Trypticase soy broth-yeast extract with the stressed cells resulted in growth that was superior to that in Listeria enrichment broth. Injured cells were fully recovered at 6 to 8 h.

MeSH terms

  • Caseins / pharmacology
  • Cold Temperature / adverse effects
  • Culture Media* / pharmacology
  • Freezing
  • Hot Temperature / adverse effects
  • Listeria monocytogenes / drug effects
  • Listeria monocytogenes / growth & development*
  • Protein Hydrolysates / pharmacology

Substances

  • Caseins
  • Culture Media
  • Protein Hydrolysates
  • trypticase-soy broth