Induction of a Streptomyces cacaoi beta-lactamase gene cloned in S. lividans

Mol Gen Genet. 1992 Oct;235(1):41-8. doi: 10.1007/BF00286179.

Abstract

The previously cloned class A beta-lactamase gene (bla) of Streptomyces cacaoi was shown to be inducible by beta-lactam compounds in the host organism S. lividans. A regulatory region of 2.75 kb was identified and the nucleotide sequence determined. It contained four open reading frames (ORFs) of which only two were complete and required for induction. ORF1-ORF2 exerted a positive regulatory effect on the expression of bla. Inactivation of ORF1 or of ORF2 resulted not only in the loss of induction, but also in a 30- to 60-fold decrease in the basal (non-induced) level of beta-lactamase production. ORF1 codes for a DNA-binding protein related to the AmpR repressor/activator, which controls the expression of ampC (class C beta-lactamase) genes in several Enterobacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Cloning, Molecular
  • Codon
  • DNA, Bacterial
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation, Bacterial / drug effects
  • Molecular Sequence Data
  • Open Reading Frames
  • Penicillins / metabolism
  • Regulatory Sequences, Nucleic Acid*
  • Sequence Homology, Amino Acid
  • Streptomyces / enzymology
  • Streptomyces / genetics*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • beta-Lactamases / genetics*
  • beta-Lactams

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Codon
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Penicillins
  • Transcription Factors
  • beta-Lactams
  • AmpR protein, Bacteria
  • beta-Lactamases

Associated data

  • GENBANK/X63780