Abstract
The previously cloned class A beta-lactamase gene (bla) of Streptomyces cacaoi was shown to be inducible by beta-lactam compounds in the host organism S. lividans. A regulatory region of 2.75 kb was identified and the nucleotide sequence determined. It contained four open reading frames (ORFs) of which only two were complete and required for induction. ORF1-ORF2 exerted a positive regulatory effect on the expression of bla. Inactivation of ORF1 or of ORF2 resulted not only in the loss of induction, but also in a 30- to 60-fold decrease in the basal (non-induced) level of beta-lactamase production. ORF1 codes for a DNA-binding protein related to the AmpR repressor/activator, which controls the expression of ampC (class C beta-lactamase) genes in several Enterobacteria.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Base Sequence
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Cloning, Molecular
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Codon
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DNA, Bacterial
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Gene Expression Regulation, Bacterial / drug effects
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Molecular Sequence Data
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Open Reading Frames
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Penicillins / metabolism
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Regulatory Sequences, Nucleic Acid*
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Sequence Homology, Amino Acid
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Streptomyces / enzymology
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Streptomyces / genetics*
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Transcription Factors / genetics
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Transcription Factors / metabolism
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beta-Lactamases / genetics*
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beta-Lactams
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Codon
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DNA, Bacterial
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DNA-Binding Proteins
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Penicillins
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Transcription Factors
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beta-Lactams
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AmpR protein, Bacteria
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beta-Lactamases