Plasmid transformation of Ruminococcus albus by means of high-voltage electroporation

P S Cocconcelli; E Ferrari; F Rossi; V Bottazzi

doi:10.1016/0378-1097(92)90631-w

Plasmid transformation of Ruminococcus albus by means of high-voltage electroporation

FEMS Microbiol Lett. 1992 Jul 15;73(3):203-7. doi: 10.1016/0378-1097(92)90631-w.

Authors

P S Cocconcelli¹, E Ferrari, F Rossi, V Bottazzi

Affiliation

¹ Istituto di Microbiologia, Universitá Cattolica del Sacro Cuore, Piacenza, Italy.

PMID: 1426982
DOI: 10.1016/0378-1097(92)90631-w

Abstract

To apply recombinant DNA techniques to the genetic manipulation of cellulolytic ruminal bacteria, a plasmid vector transformation system must be available. The objective of this work was to develop a system for plasmid transformation of Ruminococcus albus. Using high voltage electrotransformation, pSC22 and pCK17 plasmid vectors, derived from lactic acid bacteria plasmids and replicating via single-stranded DNA intermediate, were successfully introduced into three freshly isolated R. albus strains and into R. albus type strain ATCC 27210. The optimization of the electrotransformation condition raised the electroporation efficiency up to 3 x 10(5) transformants per microgram of pSC22 plasmid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Bacterial / genetics*
Genetic Vectors*
Gram-Positive Cocci / genetics*
Plasmids*
Transfection*
Transformation, Bacterial*

Substances

DNA, Bacterial