Abstract
A Gag protein segment of human immunodeficiency virus 1 (HIV-1) has been fused to a C terminally truncated core antigen of hepatitis B virus (HBcAg) using an E. coli expression system. Fusion of 90 amino acids of HIV-1 Gag protein to HBcAg still allowed the formation of capsids presenting on their surface epitopes of HIV-1 core protein, whereas fusion of 317, 189, or 100 amino acids of Gag prevented self-assembly of chimeric particles. Mice immunized with recombinant particles emulsified with Freund's complete adjuvant (CFA) or aluminium hydroxide developed high anti-HBcAg titers. However, anti-HIVp24 antibodies were detected only in mice inoculated with immunogen emulsified with CFA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AIDS Vaccines / genetics
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AIDS Vaccines / immunology
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Animals
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Blotting, Western
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Epitopes / genetics
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Epitopes / immunology
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Escherichia coli / genetics
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Freund's Adjuvant
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HIV Antibodies / biosynthesis*
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HIV Core Protein p24 / genetics
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HIV Core Protein p24 / immunology*
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HIV-1 / genetics
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HIV-1 / immunology
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Hepatitis B Antibodies / biosynthesis*
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Hepatitis B Core Antigens / genetics
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Hepatitis B Core Antigens / immunology*
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Hepatitis B Vaccines
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Hepatitis B virus / genetics
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Hepatitis B virus / immunology
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Humans
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Mice
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Microscopy, Electron
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Plasmids / genetics
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology*
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Recombinant Fusion Proteins / ultrastructure
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
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Viral Hepatitis Vaccines / genetics
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Viral Hepatitis Vaccines / immunology
Substances
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AIDS Vaccines
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Epitopes
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HIV Antibodies
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HIV Core Protein p24
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Hepatitis B Antibodies
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Hepatitis B Core Antigens
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Hepatitis B Vaccines
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Recombinant Fusion Proteins
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Vaccines, Synthetic
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Viral Hepatitis Vaccines
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Freund's Adjuvant