1H NMR study of cortex neurons and cerebellar granule cells on microcarriers and their PCA extracts: lactate production under hypoxia

Abstract

Lactate production of 6-day-old cerebral cortex neurons and 7-day-old cerebellar granule cells from mouse brain attached to cytodex 3 microcarriers was studied as a function of time, under hypoxic conditions using 1H NMR. Perchloric acid extracts of both cell types were prepared and 1H NMR spectra showed compounds characteristic for these neurons. In particular the granule cell extracts showed a large amount of glutamate as expected from biochemical experiments, whereas the cortex neurons showed a large amount of 4-aminobutyric acid.