Sugar modified oligonucleotides: synthesis, nuclease resistance and base pairing of oligodeoxynucleotides containing 1-(4'-thio-beta-D-ribofuranosyl)-thymine

Biochem Biophys Res Commun. 1992 Apr 30;184(2):797-803. doi: 10.1016/0006-291x(92)90660-d.

Abstract

XdT12 and dT5XdT6, where X is 1-(4'-thio-beta-d-ribofuranosyl)-thymine, were synthesized. The first oligonucleotide presents a better stability against calf spleen phosphodiesterase than natural dT12. The second one hybridizes with complementary sequence. However the X:A base pairing stability is lower than natural T:A one.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Composition
  • Cattle
  • Drug Stability
  • Indicators and Reagents
  • Magnetic Resonance Spectroscopy
  • Nucleic Acid Denaturation
  • Oligonucleotides / chemical synthesis*
  • Oligonucleotides / metabolism
  • Phosphoric Diester Hydrolases / metabolism*
  • Spleen / enzymology
  • Substrate Specificity
  • Thionucleosides*
  • Thymidine / analogs & derivatives*

Substances

  • Indicators and Reagents
  • Oligonucleotides
  • Thionucleosides
  • 1-(4'-thio-beta-ribofuranosyl)thymine
  • Phosphoric Diester Hydrolases
  • Thymidine