Abstract
XdT12 and dT5XdT6, where X is 1-(4'-thio-beta-d-ribofuranosyl)-thymine, were synthesized. The first oligonucleotide presents a better stability against calf spleen phosphodiesterase than natural dT12. The second one hybridizes with complementary sequence. However the X:A base pairing stability is lower than natural T:A one.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Composition
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Cattle
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Drug Stability
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Indicators and Reagents
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Magnetic Resonance Spectroscopy
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Nucleic Acid Denaturation
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Oligonucleotides / chemical synthesis*
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Oligonucleotides / metabolism
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Phosphoric Diester Hydrolases / metabolism*
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Spleen / enzymology
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Substrate Specificity
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Thionucleosides*
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Thymidine / analogs & derivatives*
Substances
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Indicators and Reagents
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Oligonucleotides
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Thionucleosides
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1-(4'-thio-beta-ribofuranosyl)thymine
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Phosphoric Diester Hydrolases
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Thymidine