Diagnosis of equid herpesviruses -1 and -4 by polymerase chain reaction

Equine Vet J. 1992 Jan;24(1):20-5. doi: 10.1111/j.2042-3306.1992.tb02772.x.

Abstract

The polymerase chain reaction (PCR) is a sensitive technique used to detect DNA of viral pathogens. We have applied the technique to the detection of Equid herpesviruses-1 and -4 (EHV-1 and EHV-4) DNA within nasopharyngeal swab samples from horses. Ninety-eight samples from suspected field cases and in-contact horses were analysed. The assays were conducted blind and later decoded and compared with virus isolation data. Our results indicate that PCR is a sensitive and rapid technique for the diagnosis of EHV-1 and EHV-4 infection.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA, Viral / analysis*
  • Herpesviridae / genetics*
  • Herpesviridae / isolation & purification
  • Herpesviridae Infections / diagnosis
  • Herpesviridae Infections / veterinary*
  • Herpesvirus 1, Equid / genetics*
  • Herpesvirus 1, Equid / isolation & purification
  • Horse Diseases / diagnosis*
  • Horses
  • Molecular Sequence Data
  • Nasopharynx / microbiology
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes / chemistry
  • Polymerase Chain Reaction
  • Sensitivity and Specificity

Substances

  • DNA, Viral
  • Oligonucleotide Probes