Immobilised enzymes: carrier-bound or carrier-free?

Curr Opin Biotechnol. 2003 Aug;14(4):387-94. doi: 10.1016/s0958-1669(03)00096-x.

Abstract

Recent advances have been made in the development of carrier-free immobilised enzymes and several criteria established for the selection of immobilised enzymes for biotransformations. The pros and cons of carrier-free versus carrier-bound immobilised enzymes and of each type of carrier-free enzyme are discussed.

Publication types

  • Review

MeSH terms

  • Catalysis
  • Catalytic Domain
  • Cross-Linking Reagents / chemistry
  • Crystallization
  • Enzyme Stability
  • Enzymes, Immobilized / chemical synthesis
  • Enzymes, Immobilized / chemistry*
  • Enzymes, Immobilized / economics
  • Kinetics
  • Microspheres
  • Polymers / chemistry
  • Protein Binding
  • Protein Conformation
  • Solubility
  • Solvents
  • Stereoisomerism
  • Substrate Specificity

Substances

  • Cross-Linking Reagents
  • Enzymes, Immobilized
  • Polymers
  • Solvents