Conditions for determination of multi-trace elements simultaneously in lychee fruit by ICP-AES. Al, Ba, Cr, Mg, Mn, Sr, Mo, Pb, Se, Zn, Fe, Cu and Ca in different parts and different growth periods of lychee fruit in Dongguan area were determined, relative standard deviation of the method is between 1.3%-4.9%, and recovery is between 89%-124%, analytical results were satisfactory. And compared the concentration and distribution of those trace elements with those in other areas. We find that the concentration of Mo in lychee fruit sample we determine is much lower, which implies that we can improve the production and quality of lychee by applying Mo to the tree. Some elements essential to human such as Zn, Fe, Mg and Ca are high in lychee fruit, implied that nutritive value of this fruit breed is higher.