Functional cloning and characterization of a multidrug efflux pump, mexHI-opmD, from a Pseudomonas aeruginosa mutant

Hiroshi Sekiya; Takehiko Mima; Yuji Morita; Teruo Kuroda; Tohru Mizushima; Tomofusa Tsuchiya

doi:10.1128/AAC.47.9.2990-2992.2003

Functional cloning and characterization of a multidrug efflux pump, mexHI-opmD, from a Pseudomonas aeruginosa mutant

Antimicrob Agents Chemother. 2003 Sep;47(9):2990-2. doi: 10.1128/AAC.47.9.2990-2992.2003.

Authors

Hiroshi Sekiya¹, Takehiko Mima, Yuji Morita, Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

Affiliation

¹ Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, 700-8530, Japan.

Abstract

We isolated mutant YM644, which showed elevated resistance to norfloxacin, ethidium bromide, acriflavine, and rhodamine 6G, from Pseudomonas aeruginosa YM64, a strain that lacks four major multidrug efflux pumps. The genes responsible for the resistance were mexHI-opmD. Elevated ethidium extrusion was observed with cells of YM644 and YM64 harboring a plasmid carrying the genes. Disruption of the genes in the chromosomal DNA of YM644 made the cells sensitive to the drugs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / metabolism
Anti-Bacterial Agents / pharmacology
Cloning, Molecular
Drug Resistance, Multiple, Bacterial / genetics
Gene Deletion
Membrane Transport Proteins / genetics*
Membrane Transport Proteins / metabolism*
Mutation / genetics*
Plasmids / genetics
Pseudomonas aeruginosa / genetics*
Pseudomonas aeruginosa / metabolism*
Tetracycline / pharmacology
Vanadium / pharmacology

Substances

Anti-Bacterial Agents
Membrane Transport Proteins
OpmD protein, Pseudomonas aeruginosa
Vanadium
Tetracycline