S-Adenosyl-L-methionine inhibitors delta(24)-sterol methyltransferase and delta(24(28))-sterol methylreductase as possible agents against Paracoccidioides brasiliensis

Antimicrob Agents Chemother. 2003 Sep;47(9):2966-70. doi: 10.1128/AAC.47.9.2966-2970.2003.

Abstract

We studied the antiproliferative effects of three azasterol analogs [piperidyl-2-yl-5alpha-pregnan-3beta,20(R)-diol (AZA-1), 22-piperidin-2-yl-pregnan-22(S),3beta-diol (AZA-2), and 22-piperidin-3-yl-pregnan-22(S),3beta-diol (AZA-3)] and their effects on the lipid composition of the pathogenic yeastlike phase of the dimorphic fungus Paracoccidioides brasiliensis. Inhibition was 100% for AZA-1 at 5 microM, 62% for AZA-2 at 10 microM, and 100% for AZA-3 at 0.5 microM. The analogs inhibited different stages of the sterol biosynthesis pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Culture Media
  • Methyltransferases / pharmacology*
  • Oxidoreductases / pharmacology*
  • Paracoccidioides / drug effects*
  • Paracoccidioides / growth & development
  • S-Adenosylmethionine / antagonists & inhibitors*
  • Structure-Activity Relationship

Substances

  • Culture Media
  • S-Adenosylmethionine
  • Oxidoreductases
  • delta(24(28))-sterol methylreductase
  • Methyltransferases
  • delta 24-sterol methyltransferase