Objective: To study lung squamamouscarcinoma and lung adenocarcinoma gene expression profiles by cDNA microarrays, compare them with those of pulmonary inflammatory pseudoneoplasma, and screen the differential expression gene and the common expression gene in these types of lung cancer in order to further study early diagnosis and treatment of lung cancer.
Methods: cDNA microarray chips containing 4096 human target genes were used to identify gene expression profiles in lung squamous cell carcinoma, adenocarcinoma, and pulmonary inflammatory pseudoneoplasma.
Results: Among the target genes, 591 differentially expressed genes were identified in lung squamous cell carcinoma; among them, there were 476 genes unexpressed in pulmonary inflammatory pseudoneoplasma; 293 of 476 differentially expressed genes were up-regulated and the other 183 were down-regulated. There were 524 differentially expressed genes in adenocarcinoma; among them, 460 genes were not expressed in pulmonary inflammatory pseudoneoplasma; 214 of the 460 differentially expressed genes were up-regulated and 246 were down-regulated. However, there were a total of 113 genes differentially expressed the both lung squamous cell carcinoma and adenocarcinoma.
Conclusion: There were distinct gene expression profiles among lung squamous cell carcinoma and adenocarcinoma. All those gene expression profiles and genes screened out provide important clues for further investigation of carcinogenesis, invasion and metastasis of non small cell lung cancer and its molecular characteristics.