Single nucleotide mismatch analysis using oligonucleotide probes synthesized on bacterial magnetic particle

Biomol Eng. 2003 Jul;20(4-6):305-9. doi: 10.1016/s1389-0344(03)00036-4.

Abstract

An approach to analyze mismatches using short and specific oligonucleotide probes directly synthesized on bacterial magnetic particles (BMPs) by phosphoramidite methods was exploited. Approximately 126 molecules of 4-mer oligonucleotides/particle were synthesized on BMPs with high reaction efficiencies. Hybridization between FITC-labeled oligonucleotides and chemically synthesized oligonucleotides on BMPs was performed. Perfect matched and mismatched hybridizations were successfully discriminated by using the oligonucleotide probes on BMPs.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Base Pair Mismatch / genetics*
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods
  • Coated Materials, Biocompatible / chemical synthesis
  • Coated Materials, Biocompatible / chemistry*
  • Gene Expression Profiling / instrumentation
  • Gene Expression Profiling / methods*
  • Membrane Proteins / chemistry*
  • Nanotechnology / instrumentation
  • Nanotechnology / methods*
  • Oligonucleotide Array Sequence Analysis / instrumentation
  • Oligonucleotide Array Sequence Analysis / methods*
  • Oligonucleotide Probes / chemical synthesis
  • Oligonucleotide Probes / chemistry*
  • Polymorphism, Single Nucleotide / genetics*

Substances

  • Bacterial Proteins
  • Coated Materials, Biocompatible
  • Membrane Proteins
  • MpsA protein, Magnetospirillum sp.
  • Oligonucleotide Probes