Abstract
An approach to analyze mismatches using short and specific oligonucleotide probes directly synthesized on bacterial magnetic particles (BMPs) by phosphoramidite methods was exploited. Approximately 126 molecules of 4-mer oligonucleotides/particle were synthesized on BMPs with high reaction efficiencies. Hybridization between FITC-labeled oligonucleotides and chemically synthesized oligonucleotides on BMPs was performed. Perfect matched and mismatched hybridizations were successfully discriminated by using the oligonucleotide probes on BMPs.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / chemistry*
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Base Pair Mismatch / genetics*
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Biosensing Techniques / instrumentation
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Biosensing Techniques / methods
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Coated Materials, Biocompatible / chemical synthesis
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Coated Materials, Biocompatible / chemistry*
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Gene Expression Profiling / instrumentation
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Gene Expression Profiling / methods*
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Membrane Proteins / chemistry*
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Nanotechnology / instrumentation
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Nanotechnology / methods*
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Oligonucleotide Array Sequence Analysis / instrumentation
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Oligonucleotide Array Sequence Analysis / methods*
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Oligonucleotide Probes / chemical synthesis
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Oligonucleotide Probes / chemistry*
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Polymorphism, Single Nucleotide / genetics*
Substances
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Bacterial Proteins
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Coated Materials, Biocompatible
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Membrane Proteins
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MpsA protein, Magnetospirillum sp.
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Oligonucleotide Probes