Differentiation of bone marrow stromal stem cells into cardiomyocyte-like cells in different mammalian species

V I Shumakov; N A Onishchenko; M E Krasheninnikov; V A Zaidenov; I V Potapov; L V Bashkina; A V Bersenev

doi:10.1023/a:1024681319084

Differentiation of bone marrow stromal stem cells into cardiomyocyte-like cells in different mammalian species

Bull Exp Biol Med. 2003 Apr;135(4):393-6. doi: 10.1023/a:1024681319084.

Authors

V I Shumakov¹, N A Onishchenko, M E Krasheninnikov, V A Zaidenov, I V Potapov, L V Bashkina, A V Bersenev

Affiliation

¹ Institute of Transplantology and Artificial Organs, Ministry of Health of Russian Federation, Moscow.

PMID: 12910318
DOI: 10.1023/a:1024681319084

Abstract

We describe the possibility of obtaining cardiomyocyte-like cell cultures from rat, guinea pig, and human bone marrow stromal stem cells. The content of troponin I-positive cells attains 35-45% of the total number of cells in the cultures and persists at this level for up to 4 months under differentiation conditions. Spontaneous contractions of cardiomyocyte-like cells were observed after the formation of cell monolayer under differentiation conditions.

MeSH terms

Animals
Bone Marrow Cells / cytology
Bone Marrow Cells / physiology*
Cell Differentiation / physiology*
Cell Size
Cells, Cultured
Culture Media
Guinea Pigs
Hematopoietic Stem Cells / cytology
Hematopoietic Stem Cells / physiology*
Humans
Myocardial Contraction
Myocytes, Cardiac / cytology
Myocytes, Cardiac / physiology*
Rats
Rats, Wistar
Stromal Cells / cytology
Stromal Cells / physiology*
Troponin I / metabolism

Substances

Culture Media
Troponin I