A novel chitosanase from Bacillus sp. strain K17: gene cloning and expression in Escherichia coli

Rie Yatsunami; Yuri Sakihama; Mamie Suzuki; Tetsuya Fukazawa; Shinji Shimizu; Tomoko Sunami; Kimiko Endo; Akio Takénaka; Satoshi Nakamura

doi:10.1093/nass/2.1.227

A novel chitosanase from Bacillus sp. strain K17: gene cloning and expression in Escherichia coli

Nucleic Acids Res Suppl. 2002:(2):227-8. doi: 10.1093/nass/2.1.227.

Authors

Rie Yatsunami¹, Yuri Sakihama, Mamie Suzuki, Tetsuya Fukazawa, Shinji Shimizu, Tomoko Sunami, Kimiko Endo, Akio Takénaka, Satoshi Nakamura

Affiliation

¹ Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.

PMID: 12903188
DOI: 10.1093/nass/2.1.227

Abstract

The gene encoding a novel chitosanase from Bacillus sp. strain K17 was cloned and sequenced. The nucleotide sequence of the gene contained an open reading frame corresponded to a protein of 453 amino acids. The deduced amino acid sequence of the K17 chitosanase exhibited the highest homology to those of family 8 glycanases, suggesting that the enzyme belonged to family 8.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacillus / enzymology*
Base Sequence
Cloning, Molecular
DNA Primers
Escherichia coli / enzymology
Glycoside Hydrolases / chemistry
Glycoside Hydrolases / genetics*
Glycoside Hydrolases / metabolism
Molecular Sequence Data
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism

Substances

DNA Primers
Recombinant Proteins
Glycoside Hydrolases
chitosanase