Patassium sensing oligonucleotide, PSO, based on DNA tetraplex formation

Takahiko Nojima; Hiroyuki Ueyama; Makoto Takagi; Shigeori Takenaka

doi:10.1093/nass/2.1.125

Patassium sensing oligonucleotide, PSO, based on DNA tetraplex formation

Nucleic Acids Res Suppl. 2002:(2):125-6. doi: 10.1093/nass/2.1.125.

Authors

Takahiko Nojima¹, Hiroyuki Ueyama, Makoto Takagi, Shigeori Takenaka

Affiliation

¹ Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka 812-8581, Japan.

PMID: 12903137
DOI: 10.1093/nass/2.1.125

Abstract

Potassium Sensing Oligonucleotide, PSO, showing a high selectivity for K+ was synthesized by connecting 6-FAM and 6-TAMRA at the 5'- and 3'-termini of d(GGG TTA GGG TTA GGG TTA GGG), respectively. Circular dichroism (CD) spectra of PSO in the absence and presence of a various amount of KCl or NaCl revealed that PSO assumes an intramolecular or intermolecular antiparallel tetraplex structure in the presence of K+ and Na+, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Circular Dichroism
DNA / chemistry*
Nucleic Acid Conformation*
Oligonucleotides / chemistry*
Potassium / metabolism*
Spectrometry, Fluorescence

Substances

Oligonucleotides
DNA
Potassium