The complete nucleotide sequence of two distinct geminiviruses infecting cucurbits in Vietnam

Arch Virol. 2003 Aug;148(8):1523-41. doi: 10.1007/s00705-003-0109-6.

Abstract

We have characterised two distinct geminiviruses that infect cucurbit cultivars in Vietnam. The genomes of both viruses consisted of two circular ssDNA components (DNA-A and DNA-B), with a genome arrangement and coding sequence typical of viruses in the Begomovirus genus in the family Geminiviridae. The sequence of DNA-A of one of the viruses was approximately 97% similar to Squash leaf curl virus-China (SLCV-Ch), for which a DNA-B has yet to be identified. We have named this virus Squash leaf curl virus-Vietnam (SLCV-Vn). The intergenic region of the SLCV-Vn DNA-B contained a 40 nt deletion between the putative AC1 TATA box and the stem loop. A second virus isolated from loofa in southern Vietnam was only 80% similar to SLCV-Vn over the complete DNA-A sequence, however the nucleotide sequence in the coat protein coding regions was 95% similar. We have named this virus Loofa yellow mosaic virus-Vietnam (LYMV-Vn). Other regions of the SLCV-Vn and LYMV-Vn genomes differed markedly, suggesting the coat protein coding region was recombinant. The DNA-B of both viruses were only 60% similar over the complete nucleotide sequence, although the encoded amino acid sequence of the BC1 gene was 90% identical.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cucurbita / virology*
  • DNA, Intergenic / analysis
  • DNA, Viral / analysis
  • DNA, Viral / genetics
  • Geminiviridae / classification*
  • Geminiviridae / genetics
  • Geminiviridae / isolation & purification
  • Genetic Variation
  • Genome, Viral*
  • Molecular Sequence Data
  • Phylogeny
  • Plant Diseases / virology
  • Plant Leaves / virology
  • Recombination, Genetic
  • Sequence Analysis, DNA*
  • Vietnam

Substances

  • DNA, Intergenic
  • DNA, Viral

Associated data

  • GENBANK/AF509739
  • GENBANK/AF509740
  • GENBANK/AF509741
  • GENBANK/AF509742
  • GENBANK/AF509743