Grape seeds: the best lignocellulosic waste to produce laccase by solid state cultures of Trametes hirsuta

Biotechnol Lett. 2003 Mar;25(6):491-5. doi: 10.1023/a:1022660230653.

Abstract

Grape seeds were used by Trametes hirsuta as a substrate for laccase production giving 23 kU l(-1), which was 10-fold the value attained in the cultures with no lignocellulosic waste addition. The dyes, Indigo Carmine and Bromophenol Blue, were easily decolourised (100% in 24 h) by the extracellular liquid obtained in such cultures, whereas Methyl Orange (65% in 24 h) and Phenol Red (36% in 24 h) were more resistant to degradation. This shows the specificity of laccase towards different dye structures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods*
  • Cellulose / chemistry
  • Cellulose / metabolism*
  • Color
  • Coloring Agents / chemistry
  • Copper Sulfate / pharmacology
  • Hordeum / chemistry
  • Laccase
  • Lignin / chemistry
  • Lignin / metabolism*
  • Oxidoreductases / biosynthesis*
  • Polyporales / drug effects
  • Polyporales / metabolism*
  • Quality Control
  • Refuse Disposal / methods
  • Seeds / chemistry
  • Seeds / metabolism
  • Substrate Specificity
  • Vitamins / pharmacology
  • Vitis / chemistry*
  • Vitis / metabolism

Substances

  • Coloring Agents
  • Vitamins
  • lignocellulose
  • Cellulose
  • Lignin
  • Oxidoreductases
  • Laccase
  • Copper Sulfate