Abstract
Grape seeds were used by Trametes hirsuta as a substrate for laccase production giving 23 kU l(-1), which was 10-fold the value attained in the cultures with no lignocellulosic waste addition. The dyes, Indigo Carmine and Bromophenol Blue, were easily decolourised (100% in 24 h) by the extracellular liquid obtained in such cultures, whereas Methyl Orange (65% in 24 h) and Phenol Red (36% in 24 h) were more resistant to degradation. This shows the specificity of laccase towards different dye structures.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cell Culture Techniques / methods*
-
Cellulose / chemistry
-
Cellulose / metabolism*
-
Color
-
Coloring Agents / chemistry
-
Copper Sulfate / pharmacology
-
Hordeum / chemistry
-
Laccase
-
Lignin / chemistry
-
Lignin / metabolism*
-
Oxidoreductases / biosynthesis*
-
Polyporales / drug effects
-
Polyporales / metabolism*
-
Quality Control
-
Refuse Disposal / methods
-
Seeds / chemistry
-
Seeds / metabolism
-
Substrate Specificity
-
Vitamins / pharmacology
-
Vitis / chemistry*
-
Vitis / metabolism
Substances
-
Coloring Agents
-
Vitamins
-
lignocellulose
-
Cellulose
-
Lignin
-
Oxidoreductases
-
Laccase
-
Copper Sulfate