Computer-assisted analysis of the structure of regular branched polysaccharides containing 2,3-disubstituted rhamnopyranose and mannopyranose residues on the basis of 13C NMR data

G M Lipkind; A S Shashkov; N E Nifant'ev; N K Kochetkov

doi:10.1016/s0008-6215(92)84229-l

Computer-assisted analysis of the structure of regular branched polysaccharides containing 2,3-disubstituted rhamnopyranose and mannopyranose residues on the basis of 13C NMR data

Carbohydr Res. 1992 Dec 31:237:11-22. doi: 10.1016/s0008-6215(92)84229-l.

Authors

G M Lipkind¹, A S Shashkov, N E Nifant'ev, N K Kochetkov

Affiliation

¹ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.

PMID: 1284111
DOI: 10.1016/s0008-6215(92)84229-l

Abstract

A computer-assisted approach to the analysis of the structure of branched polysaccharides that contain 2,3-di-O-glycosylated alpha-rhamnopyranose and alpha-mannopyranose residues is based on evaluation of the 13C NMR spectra, using glycosylation effects and their deviations from additivity (delta delta values) at the branch points. This approach, in combination with monosaccharide and methylation analysis data, has been verified on a series of bacterial polysaccharides of known structure.

Publication types

Comparative Study

MeSH terms

Carbohydrate Sequence
Computer Simulation
Enterobacteriaceae / chemistry*
Escherichia coli / chemistry
Magnetic Resonance Spectroscopy
Mannosides / chemistry*
Molecular Sequence Data
O Antigens
Polysaccharides, Bacterial / chemistry*
Pseudomonas / chemistry*
Pseudomonas aeruginosa / chemistry
Rhamnose / analogs & derivatives*
Shigella flexneri / chemistry
Software
Stereoisomerism

Substances

Mannosides
O Antigens
Polysaccharides, Bacterial
Rhamnose