Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum

Yoshiko Okamura; Haruko Takeyama; Takumi Sekine; Toshifumi Sakaguchi; Aris Tri Wahyudi; Rika Sato; Shinji Kamiya; Tadashi Matsunaga

doi:10.1128/AEM.69.7.4274-4277.2003

Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum

Appl Environ Microbiol. 2003 Jul;69(7):4274-7. doi: 10.1128/AEM.69.7.4274-4277.2003.

Authors

Yoshiko Okamura¹, Haruko Takeyama, Takumi Sekine, Toshifumi Sakaguchi, Aris Tri Wahyudi, Rika Sato, Shinji Kamiya, Tadashi Matsunaga

Affiliation

¹ Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Koganei, Tokyo.

Abstract

A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Cloning, Molecular
Conjugation, Genetic
Electroporation
Escherichia coli / genetics
Escherichia coli / metabolism
Genetic Vectors*
Plasmids / genetics*
Replicon
Rhodospirillaceae / genetics*