Improving quality and harvest period of protein crystals for structure-based drug design: effects of a gel and a magnetic field on bovine adenosine deaminase crystals

Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1333-5. doi: 10.1107/s0907444903008187. Epub 2003 Jun 27.

Abstract

The overall crystal quality as well as the harvest period of bovine adenosine deaminase containing a zinc ion at the active centre has been compared in three different environments: crystallization as a control, crystallization with agarose gel and crystallization in a high magnetic field. In crystallization with agarose gel, the probability of obtaining high-quality crystals was somewhat increased, but the harvest period was elongated. On the other hand, in crystallization in a magnetic field, the probability of obtaining high-quality crystals was greatly increased. Furthermore, the harvest period for crystal growth in a magnetic field was much shorter than that with agarose gel.

Publication types

  • Comparative Study

MeSH terms

  • Adenosine Deaminase / chemistry*
  • Animals
  • Cattle
  • Crystallization / methods
  • Crystallization / standards*
  • Crystallography, X-Ray
  • Drug Design*
  • Gels
  • Magnetics
  • Proteins / chemistry
  • Sepharose

Substances

  • Gels
  • Proteins
  • Sepharose
  • Adenosine Deaminase