Ratio of loading dye to PCR product affects sensitivity of single-stranded conformational polymorphism analysis

Biotechniques. 2003 Jun;34(6):1168-70. doi: 10.2144/03346bm09.

Authors

Jelena Tesic¹, Zorica Lepsanovic, Veljko Mirovic

Affiliation

¹ Military Medical Academy, Belgrade, Yugoslavia.

PMID: 12813883
DOI: 10.2144/03346bm09

No abstract available

MeSH terms

Biotechnology
Coloring Agents
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification
DNA-Directed RNA Polymerases / genetics
Drug Resistance, Bacterial / genetics
Genes, Bacterial
Mycobacterium tuberculosis / drug effects
Mycobacterium tuberculosis / genetics
Polymerase Chain Reaction / methods*
Polymerase Chain Reaction / statistics & numerical data
Polymorphism, Single-Stranded Conformational*
Rifampin / pharmacology
Sensitivity and Specificity

Substances

Coloring Agents
DNA, Bacterial
DNA-Directed RNA Polymerases
RNA polymerase beta subunit
Rifampin